鸭源鸡杆菌对HeLa细胞的黏附和侵袭特性

doi:10.11843/j.issn.0366-6964.2015.02.017

Abstract

Abstract:

In order to determine the cell adhesion and invasion characteristics of Gallibacterium anatis（G.anatis），an in vitro HeLa cell model was established.The adhesion and invasion characteristics of 2 strains were evaluated by means of adhesion and invasion experiment，Gram and Giemsa staining，Scanning electron microscopy and Transmission electron microscopy.Adhesion counting assay demonstrated that the high adhesive of G.anatis Yu-PDS-RZ-1-SLG strain and adhering bacteria numbers were 0.84•cell^－1，4.68•cell^－1，8.52•cell^－1，9.27•cell^－1 and 8.2 •cell^－1 at 30，60，90，120，150 min after infection，respectively.G.anatis F149^Tstrain revealed a weak adhesive level.Invasion assay showed weak invasive of G.anatis Yu-PDS-RZ-1-SLG strain and no invasion of G.anatis F149^Tstrain，and invading bacteria numbers of G.anatis Yu-PDS-RZ-1-SLG strain reached the maximum at 150 min，about 8.27 per 1 000 cells，but cell disruption，cell activity weakened subsequently.Staining results and electron microscopy experiments further confirmed that G.anatis Yu-PDS-RZ-1-SLG strain adhere to surface and invade Hela cells.Adherence was prevented by treating bacterial cells with trypsin，suggesting the participation of proteins in this process.The adhesive and invasive capacity could be an important ability for colonization of tissue surfaces of G.anatis.The establishment of cell model provided the basis for further research on pathogenic mechanism of G.anatis.

CLC Number:

S852.612

ZHANG Xiu-ping，CHEN Lu，ZHAO Jun，WANG Chuan-qing，LI Yong-tao，LIU Hong-ying，PENG Zhi-feng，LU Cai-jing，JING Wen-xian，YANG Xia. Adhesion and Invasion Characteristics of Gallibacterium anatis to HeLa Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 295-302.

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Adhesion and Invasion Characteristics of Gallibacterium anatis to HeLa Cells

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